From: Charles Plessy <plessy@riken.jp>
Date: Thu, 19 Apr 2018 01:55:14 +0000 (+0900)
Subject: TdT.
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=fd62203a0e88ae6943da75284b18761ef7dbd2c7;p=source%2F.git

TdT.
---

diff --git a/biblio/11252793.mdwn b/biblio/11252793.mdwn
new file mode 100644
index 00000000..d2c2f1ec
--- /dev/null
+++ b/biblio/11252793.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Reverse transcriptase adds nontemplated nucleotides to cDNAs during 5'-RACE and primer extension."]]
+[[!tag reverse_transcription]]
+
+Biotechniques. 2001 Mar;30(3):574-80, 582
+
+Chen D, Patton JT.
+
+Reverse transcriptase adds nontemplated nucleotides to cDNAs during 5'-RACE and primer extension.
+
+[[!pmid 11252793 desc="Terminal desoxynucleotidyl transferase activity of reverse transcriptases MMLV and AMV: preference for adding As."]]
diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index fcc03633..3c53de4f 100644
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -5,13 +5,13 @@ The reverse transcriptase
 
 _(redaction in progress)_
 
-## Additives that increase reaction performance:
+### Additives that increase reaction performance
 
  - Actinomycin D ([[Perocchi et al., 2007|biblio/17897965]]).
  - [[T4 bacteriophage gene 32 protein|ssbp]] (T4gp32, [[Kenzelmann _et al._, 2004|biblio/15028277]],
    [[Piché _et al._, 2005|biblio/16461948]]).
 
-## DNA-dependent DNA polymerase activity.
+### DNA-dependent DNA polymerase activity
 
  - It is utilised in [[template_switching]] methods to add linkers to first-strand cDNAs.
  - It is also a source of antisense artefacts when the RT makes a second-strand cDNA
@@ -19,17 +19,22 @@ _(redaction in progress)_
    but not RNA-dependent polymerase activity and is used to suppress these
    artefacts ([[Perocchi et al., 2007|biblio/17897965]], [[Kanamori-Katayama et al., 2011|biblio/21596820]]).
 
-## Reverse-transcriptases tolerate terminal mismatches:
+### Terminal desoxynucleotidyl transferase (TdT) activity
+
+ - [[Chen et al., 2001|biblio/11252793]] reported a TdT activity for MMLV and
+   AMV, with a preference for adding As.
+
+### Reverse-transcriptases tolerate terminal mismatches
 
  - Reported by [[Mizuno et al., 1999|biblio/9973624]].
- - Utilised in [[Arnaud et al., 2015|biblio/27071605]] to reduce priming or
+ - Utilised in [[Arnaud et al., 2016|biblio/27071605]] to reduce priming or
    ribosomal or hemoglobin RNA.
 
-## Reverse-transcription primers:
+### Reverse-transcription primers
 
  - "N15" random pentadecamers: [[Stangegaard et al., 2006|biblio/16708763]].
  - Multi-targeted primers (MTP): [[Adomas et al., 2010|biblio/20716356]].
  - "Not-so random" (NSR) primers: [[Armour et al., 2009|biblio/19668204]].
- - "Pseudo-random" primers: [[Arnaud et al., 2015|biblio/27071605]].
+ - "Pseudo-random" primers: [[Arnaud et al., 2016|biblio/27071605]].
 
 [[!inline pages="tagged(reverse_transcription)" actions="no" limit=0]]