From: Charles Date: Wed, 9 Dec 2020 05:21:46 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=fc0013e10e1352af5ebeeaefd7995227d7102686;p=source.git Café --- diff --git a/biblio/32661437.mdwn b/biblio/32661437.mdwn new file mode 100644 index 00000000..1f0a37b6 --- /dev/null +++ b/biblio/32661437.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Automated design of thousands of nonrepetitive parts for engineering stable genetic systems."]] +[[!tag synthetic]] + +Hossain A, Lopez E, Halper SM, Cetnar DP, Reis AC, Strickland D, Klavins E, Salis HM. + +Nat Biotechnol. 2020 Dec;38(12):1466-1475. doi: 10.1038/s41587-020-0584-2 + +Automated design of thousands of nonrepetitive parts for engineering stable genetic systems. + +[[!pmid 32661437 desc="To avoid homologous recombinations, synthetic parts of a genome that have the same function (promoter, ...) need to have a different sequence. Constructing a set of functional synthetic sequences that never share k-mers of a given length (10 to 20) is difficult because the graph of related sequences is very dense. Therefore, the authors have optimised an algorithm for very dense graph."]] diff --git a/tags/automation.mdwn b/tags/automation.mdwn index 082ef04b..fd4fcf0b 100644 --- a/tags/automation.mdwn +++ b/tags/automation.mdwn @@ -8,4 +8,6 @@ Computational planning of the synthesis of complex natural products. ([[Mikulak- The use of laboratory automation in synthetic biology studied by a sociologist: [[Meckin 2020|biblio/32904024]]. +Synthetic sequences that have the same function in a genome need to differ from each other, to prevent from spurious homologous recombinations. Hossain and coll ([[2020|biblio/32661437]]) optimised an algorithm for producing libraries of "nonrepetitive" elements such as promoters. + [[!inline pages="tagged(automation)" limit=0]]