From: Charles Plessy <charles.plessy@oist.jp>
Date: Fri, 22 Oct 2021 07:35:05 +0000 (+0900)
Subject: Café
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=fa911415e579714952a9d6822455276ed635e2b8;p=source.git

Café
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+[[!meta title="Direct observation of independently moving replisomes in Escherichia coli."]]
+[[!tag bacteria replication]]
+
+Japaridze A, Gogou C, Kerssemakers JWJ, Nguyen HM, Dekker C.
+
+Nat Commun. 2020 Jun 19;11(1):3109. doi:10.1038/s41467-020-16946-7
+
+Direct observation of independently moving replisomes in _Escherichia coli_.
+
+[[!pmid 32561741 desc="Replisomes were visualised with labelling the the β-clamp (DnaN).  The chromosome was labelled by attaching a mYpet fluorescent tag to the HU protein.  A temperature-sensitive DnaC allele was used to synchronise the cell cycles and to elongate the cells.  A MreB mutation was used to generate wider cells."]]