From: Charles Plessy Date: Wed, 11 Apr 2018 01:12:49 +0000 (+0900) Subject: Add keyword. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=f948a2b964c329ad6292587c388b7c0a7a314f6d;p=source%2F.git Add keyword. --- diff --git a/biblio/29434199.mdwn b/biblio/29434199.mdwn index 46e40f05..2dd301e0 100644 --- a/biblio/29434199.mdwn +++ b/biblio/29434199.mdwn @@ -7,4 +7,4 @@ Hayashi T, Ozaki H, Sasagawa Y, Umeda M, Danno H, Nikaido I. Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs. -[[!pmid 29434199 desc="First-strand cDNAs are synthesised with a RNAse H minus reverse-transcriptase. DNAse I introduces nicks that prime synthesis of new cDNA molecules. T4gp32 promotes the strand-displacement activity of the reverse-transcriptase. Second-strand cDNAs are synthethised with Klenow fragment (3′ → 5′ exo-) primed with NSRs. In single cells, genomic DNA needs to be removed because of the DNAseI digestion during RT and the low-complexity priming of the second-strand synthesis with NSRs. The resulting DNA molecules are tagmented and sequenced with standard methods. Thus, the method is non-stranded. Despite the use of NSRs, the rRNA rate stays between 20 and 30 %."]] +[[!pmid 29434199 desc="RamDA-seq. First-strand cDNAs are synthesised with a RNAse H minus reverse-transcriptase. DNAse I introduces nicks that prime synthesis of new cDNA molecules. T4gp32 promotes the strand-displacement activity of the reverse-transcriptase. Second-strand cDNAs are synthethised with Klenow fragment (3′ → 5′ exo-) primed with NSRs. In single cells, genomic DNA needs to be removed because of the DNAseI digestion during RT and the low-complexity priming of the second-strand synthesis with NSRs. The resulting DNA molecules are tagmented and sequenced with standard methods. Thus, the method is non-stranded. Despite the use of NSRs, the rRNA rate stays between 20 and 30 %."]]