From: Charles Plessy Date: Mon, 6 Nov 2017 04:51:34 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=f78b258e4388bd841c24e6e0ddc89d9e1365a985;p=source.git Old --- diff --git a/biblio/16308152.mdwn b/biblio/16308152.mdwn new file mode 100644 index 00000000..a64c86b0 --- /dev/null +++ b/biblio/16308152.mdwn @@ -0,0 +1,10 @@ +[[!meta title="RNA amplification strategies for small sample populations."]] +[[!tag method amplification template_switching]] + +Methods. 2005 Nov;37(3):229-37 doi:10.1016/j.ymeth.2005.09.003 + +Ginsberg SD + +RNA amplification strategies for small sample populations. + +[[!pmid 16308152 desc="Detailed protocol for Terminal Continuation (TC)."]] diff --git a/biblio/To_Do b/biblio/To_Do index 51e46736..7fee4311 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -32,9 +32,6 @@ ggcacgcga/cc => C-box for dro hairy. 10537171 Full in situ random priming cDNA synthesis -9883850 [amplification] -3 rounds of T7 RNA amplification (aRNA). - 12593794 [general] Noise due to RNA extraction is 40 times greater than noise due to replicate hybridisation @@ -539,9 +536,6 @@ Unlike mouse ngn3, zebrafish ngn3 is not expressed in the pancreas (but zebrafis 16280998 [patents] Perpetual-motion machines are not patentable. -16308152 [amplification] -Detailed protocol for Terminal Continuation (TC). - 16224006 [IP] 20 % of the human coding genome is patented. diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn index 79fa05a2..370aa198 100644 --- a/tags/template_switching.mdwn +++ b/tags/template_switching.mdwn @@ -2,7 +2,10 @@ (work in progress) - - In the "_terminal continuation_" method, [[Che et al., 2004|biblio/14647400]] - essentially do template switching with DNA oligonucleotides ending in `CCC`. + - The "_terminal continuation_" method ([[Ginsberg et al., + 2002|biblio/12462399]], [[Che et al., 2004|biblio/14647400]]) is essentially + a template switching with DNA oligonucleotides ending in `CCC` or `GGG`. `AAA` + and `TTT` were also tested. An extensive protocol was published in + [[Ginsberg, 2005|biblio/16308152]]. [[!inline pages="tagged(template_switching)" limit=0]]