From: Charles Plessy <plessy@riken.jp>
Date: Thu, 12 Oct 2017 00:40:54 +0000 (+0900)
Subject: Expand.
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=f64a07cab21d4a4e76acebcc292f1f8cd572e4f0;p=source.git

Expand.
---

diff --git a/biblio/16233792.mdwn b/biblio/16233792.mdwn
index 454035de..e0edd5a6 100644
--- a/biblio/16233792.mdwn
+++ b/biblio/16233792.mdwn
@@ -1,3 +1,10 @@
 [[!meta title="Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion."]]
-[[!tag emulsion]]
+[[!tag emulsion reverse_transcription]]
+
+J Biosci Bioeng. 2005 Mar;99(3):293-5. doi:10.1263/jbb.99.293
+
+Nakano M, Nakai N, Kurita H, Komatsu J, Takashima K, Katsura S, Mizuno A.
+
+Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion.
+
 [[!pmid 16233792 desc="The emulsion is destroyed by centrifugation after 13 cycles, and then the PCR is restarted for 30 cycles."]]
diff --git a/tags/emulsion.mdwn b/tags/emulsion.mdwn
index df1139ed..5e2895f5 100644
--- a/tags/emulsion.mdwn
+++ b/tags/emulsion.mdwn
@@ -4,5 +4,9 @@ How to add contents to droplets ?
 
  - In [[Agresti et al., 2005|biblio/16260754]], small molecules mixed with EtOH or DMSO were added by vortexing.
 
+How to break the droplets ?
+
+ - In [[Nakano _et al._, 2005|biblio/16233792]], by centrifugation (oil: silicone; superfactant: TritonX-100 0.1%).
+ - In [[Agresti et al., 2005|biblio/16260754]], by centrifugation in presence of diethyl ether (oil: mineral; superfactant: TritonX-100 0.1% and Span 80 4.5%).
 
 [[!inline pages="tagged(emulsion)" actions="no" feedshow=0]]