From: Charles Plessy Date: Tue, 4 Sep 2018 08:28:25 +0000 (+0900) Subject: Old. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=f26fb7fe3f641f8d7a367e9759fa8063974a20f2;p=source%2F.git Old. --- diff --git a/biblio/30154186.mdwn b/biblio/30154186.mdwn new file mode 100644 index 00000000..ecbc8a1e --- /dev/null +++ b/biblio/30154186.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Mapping 3D genome organization relative to nuclear compartments using TSA-Seq as a cytological ruler"]] +[[!tag method sequence_tag nucleus]] + +J Cell Biol. 2018 Aug 28. pii: jcb.201807108. doi:10.1083/jcb.201807108 + +Chen Y, Zhang Y, Wang Y, Zhang L, Brinkman EK, Adam SA, Goldman R, van Steensel B, Ma J, Belmont AS. + +Mapping 3D genome organization relative to nuclear compartments using TSA-Seq as a cytological ruler + +[[!pmid 30154186 desc="Tyramide free radicals can label DNA directly. In TSA-Seq (Tyramide Signal Amplification-Sequencing), HRP is targeted on proteins of interest, biotinylated TSA labels neighboring DNA, and biotin-DNA complexes are purified and sequenced. Distances between chromosome domains and nuclear speckles is reproducible across experiments. Between peripheral lamina (marked by lamin A and B) and nuclear speckles (marked by SON), an increasing gradient of transcriptional activity is clearly visible."]]