From: Charles Date: Fri, 14 Feb 2020 03:50:36 +0000 (+0900) Subject: café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=f1b07d27cf9c9f58aab36273892e8a0e077ad54d;p=source.git café --- diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index adb34865..51efdcd8 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -71,13 +71,9 @@ nucleotide as a template. 2017b|biblio/28747695]]). -### The 5′ cap enhances C-tailing - - - [[Schmidt & Mueller, 1999|biblio/10518626]] showed that extra cytosine are - more frequently added in presence of the 5′ cap. +### Reverse-transcription of the 5′ cap. - -### The 5′ cap is reverse-transcribed +#### Pro: - [[Hirzmann et al., 1993|biblio/8346046]] observed the presence of an extra G at the 5′ end of cDNA clones, and concluded that the cap can be @@ -91,6 +87,9 @@ nucleotide as a template. reverse-transcribed, with a frequency of 10 %. They also discussed if it could be that the cap was reverse-transcribed. + - [[Schmidt & Mueller, 1999|biblio/10518626]] showed that extra cytosine are + more frequently added in presence of the 5′ cap. + - [[Ohtake et al, 2004|biblio/15500255]] synthethised RNAs with A-caps and showed that they are reverse-transcribed as Ts. @@ -100,13 +99,20 @@ nucleotide as a template. - [[Zhang et al, 2017|biblio/28673998]] published a structure of an RNA-GpppG complex that suggests that a m7GpppNm / DNA duplex could form during the - reverse-transcription of the cap + reverse-transcription of the cap. + +#### Con: - - Nevertheless, in [[Dallmeier and Neyts, 2013|biblio/23123427]], where + - [[Chenchick and coll., 1998|biblio/Chenchick_1998]] reported that non-capped + oligonucleotides were extended with long (1~5) cytosine tails, and that the + presene of a cap did not have a “significant influence”. + + - In [[Dallmeier and Neyts, 2013|biblio/23123427]], where first-strand cDNAs prepared with a phosphorylated reverse-transcription primer were circularised, amplified and sequenced, there is not visible evidence for G addition. + ### Reverse-transcriptases tolerate terminal mismatches - Reported by [[Mizuno et al., 1999|biblio/9973624]].