From: Charles Plessy Date: Mon, 19 Mar 2018 08:37:55 +0000 (+0900) Subject: Biblio X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=ee69a2db8536e3702ad7dc31bbcb7fc989a13320;p=source.git Biblio --- diff --git a/biblio/24922482.mdwn b/biblio/24922482.mdwn new file mode 100644 index 00000000..1e10e5c1 --- /dev/null +++ b/biblio/24922482.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Capture and Amplification by Tailing and Switching (CATS). An ultrasensitive ligation-independent method for generation of DNA libraries for deep sequencing from picogram amounts of DNA and RNA."]] +[[!tag template_switching amplification method]] + +RNA Biol. 2014;11(7):817-28. doi:10.4161/rna.29304 + +Turchinovich A, Surowy H, Serva A, Zapatka M, Lichter P, Burwinkel B. + +Capture and Amplification by Tailing and Switching (CATS). An ultrasensitive ligation-independent method for generation of DNA libraries for deep sequencing from picogram amounts of DNA and RNA. + +[[!pmid 24922482 desc="Pre-treatment by T4 PNK was needed when using circulating plasma RNA, probably because of phosphate or cyclo-phosphates at the 3′ end (caused by RNA cleavage). RT primer concentration scaled down according to the RNA amoounts (0.1 µM for 1 ng and 0.1 nM for 5 pg). TSO concentration: 1 µM."]]