From: Charles Plessy Date: Wed, 18 Oct 2017 01:24:07 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=ea72e5b9f2ca73937aa366d78ef7756ea3349f36;p=source.git Old --- diff --git a/biblio/9661199.mdwn b/biblio/9661199.mdwn new file mode 100644 index 00000000..00516bb1 --- /dev/null +++ b/biblio/9661199.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Man-made cell-like compartments for molecular evolution."]] +[[!tag emulsion]] + +Nat Biotechnol. 1998 Jul;16(7):652-6 doi:10.1038/nbt0798-652 + +Tawfik DS, Griffiths AD. + +Man-made cell-like compartments for molecular evolution. + +[[!pmid 9661199 desc="Only the constructs encoding methylases escape degradation."]] diff --git a/biblio/To_Do b/biblio/To_Do index e4a5d1f3..5dc84771 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -574,9 +574,6 @@ Unlike mouse ngn3, zebrafish ngn3 is not expressed in the pancreas (but zebrafis 16280998 [patents] Perpetual-motion machines are not patentable. -99661199 [emulsion] -Only the constructs encoding methylases escape degradation. - 16308152 [amplification] Detailed protocol for Terminal Continuation (TC). diff --git a/tags/emulsion.mdwn b/tags/emulsion.mdwn index 3de02b1a..b72e5ee5 100644 --- a/tags/emulsion.mdwn +++ b/tags/emulsion.mdwn @@ -7,16 +7,16 @@ How to add contents to droplets ? How to break the droplets ? - - In [[Nakano _et al._, 2005|biblio/16233792]], by centrifugation (oil: silicone; superfactant: TritonX-100 0.1%). - - In [[Agresti et al., 2005|biblio/16260754]], by centrifugation in presence of diethyl ether (oil: mineral; superfactant: TritonX-100 0.1% and Span 80 4.5%). + - In [[Nakano _et al._, 2005|biblio/16233792]], by centrifugation (oil: silicone; surfactant: Triton X-100 0.1%). + - In [[Agresti et al., 2005|biblio/16260754]], by centrifugation in presence of diethyl ether (oil: mineral; surfactant: Triton X-100 0.1% and Span 80 4.5%). - High salt: - - In [[Dielh _et al._, 2006|biblio/16791214]], by pipetting up and down in 100 mM NaCl, 1 % SDS, 1 % TritonX-100 and Tris (oil: mineral, superfactant: ABIL WE09). - - In [[Kojima _et al._, 2005|biblio/16214800]], by mixing with 1M NaCl, 5 mM Tris-HCl pH 8.0, 0.5 mM EDTA (oil: mineral; superfactant: Sun Soft No. 818SK). - - Extraction of beads with hexane:oil 1:1, in [[Levy _et al._, 2005|biblio/16131588]] (oil: mineral, superfactant: 5.5% Span 80, 0.5% Tween 80, 0.1 % TritonX-100). + - In [[Dielh _et al._, 2006|biblio/16791214]], by pipetting up and down in 100 mM NaCl, 1 % SDS, 1 % Triton X-100 and Tris (oil: mineral, surfactant: ABIL WE09). + - In [[Kojima _et al._, 2005|biblio/16214800]], by mixing with 1M NaCl, 5 mM Tris-HCl pH 8.0, 0.5 mM EDTA (oil: mineral; surfactant: Sun Soft No. 818SK). + - Extraction of beads with hexane:oil 1:1, in [[Levy _et al._, 2005|biblio/16131588]] (oil: mineral, surfactant: 5.5% Span 80, 0.5% Tween 80, 0.1 % Triton X-100). -Different kinds of superfactants: +Different kinds of surfactants: - - TritonX-100, Span 80, Tween 80 (many articles), + - Span 80 4.5 % and Tween 80 0.5 % (oil: mineral; primary paper: [[Tawfik _et al._, 1998|biblio/9661199]], followed by many others, sometimes adding Triton X-100 and changing the concentrations). - ABIL WE09 (polysiloxane–polycetyl–polyethylene glycol copolymer; primary paper: [[Dielh _et al._, 2004|biblio/14990785]]) - Sun Soft No. 818SK (polyglycerol esters of intersesterified ricinoleic acid; primary paper: [[Kojima _et al._, 2005|biblio/16214800]]) - DC 5225C Formulation Aid / DC 749 Fluid (Dow Chemical Co.) [[Margulies _et al._, 2005|biblio/16056220]]