From: Charles Plessy Date: Mon, 7 Mar 2022 04:47:42 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=e7f59f8249430d9860ead5229ac2643b383fdfd8;p=source%2F.git Café --- diff --git a/biblio/35239377.mdwn b/biblio/35239377.mdwn new file mode 100644 index 00000000..2a385643 --- /dev/null +++ b/biblio/35239377.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Transcriptional neighborhoods regulate transcript isoform lengths and expression levels."]] +[[!tag yeast synthetic variants]] + +Brooks AN, Hughes AL, Clauder-Münster S, Mitchell LA, Boeke JD, Steinmetz LM. + +Science. 2022 Mar 4;375(6584):1000-1005. doi: 10.1126/science.abg0162 + +Transcriptional neighborhoods regulate transcript isoform lengths and expression levels. + +[[!pmid 35239377 desc="“a synthetic yeast genome (Sc2.0) was designed to encode a Cre-dependent system known as synthetic chromosome rearrangement and modification by LoxP-mediated evolution (SCRaMbLE), which can generate stochastic genomic rearrangements on demand directly in its genome. These rearrangements occur at 34 base pair (bp)–loxPsym sites inserted 3 bp downstream of the stop codon of all nonessential CDSs.”"]] diff --git a/tags/synthetic.mdwn b/tags/synthetic.mdwn index 06d8d9a3..009c3613 100644 --- a/tags/synthetic.mdwn +++ b/tags/synthetic.mdwn @@ -8,4 +8,6 @@ Sc3.0 roadmap: [[Dai and coll., 2020|biblio/32791980]]. Example of neochromosome synthesis in yeast: [[Postma and coll., 2021|biblio/33423048]]. +Artificial genome scrambling in yeast 2.0 with loxPsym sites: [[Brooks and coll, 2022|biblio/35239377]]. + [[!inline pages="tagged(synthethic)" limit=0]]