From: admin Date: Mon, 2 Nov 2020 03:50:46 +0000 (+0000) Subject: updated PO files X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=e7b518d7a75d3951def0a3eaafaa189ec61e1b38;p=source.git updated PO files --- diff --git a/open-source-biologist.en.po b/open-source-biologist.en.po index 1788c12c..ee50f858 100644 --- a/open-source-biologist.en.po +++ b/open-source-biologist.en.po @@ -7,7 +7,7 @@ msgid "" msgstr "" "Project-Id-Version: PACKAGE VERSION\n" -"POT-Creation-Date: 2020-06-16 01:18+0000\n" +"POT-Creation-Date: 2020-11-02 03:50+0000\n" "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n" "Last-Translator: FULL NAME \n" "Language-Team: LANGUAGE \n" @@ -28,10 +28,9 @@ msgid "" "enhancers ([Blader and coll., 2003](https://pubmed.gov/12559493)) and their " "evolutionary conservation ([Plessy and coll., 2005](https://pubmed." "gov/15797614)). This gave me a strong interest for whole-transcriptome " -"analysis and technology. For that purpose, I have joined RIKEN in 2004, " -"where have worked on high-throughput methods for **profiling promoters and " -"inferring gene networks**, and in particular on CAGE (Cap Analysis Gene " -"Expression)." +"analysis and technology. For that purpose, I have worked at RIKEN in 2004–18 " +"on high-throughput methods for **profiling promoters and inferring gene " +"networks**, and in particular on CAGE (Cap Analysis Gene Expression)." msgstr "" #. type: Plain text @@ -47,25 +46,26 @@ msgid "" "gov/23180801)), combining multiple cap-enrichment steps ([Batut and coll., " "2013](https://pubmed.gov/22936248)), benchmarking the use of locked nucleic " "acids for template switching ([Harbers and coll., 2013](https://pubmed." -"gov/24079827)), and reducing the number of primer artefacts and unwanted " +"gov/24079827)), reducing the number of primer artefacts and unwanted " "sequences generated by ribosomal RNAs using low-complexity “pseudo-random” " "reverse-transcription primers ([Arnaud and coll., 2016](https://pubmed." -"gov/27071605))." +"gov/27071605)), and screening for optimal parameters of the template-" +"switching reaction ([Poulain and coll., 2020](https://pubmed.gov/32025730))." msgstr "" #. type: Plain text msgid "" -"On April 2013, I started a new development cycle as the leader of the " -"Genomics Miniaturization Technology Unit at RIKEN Center for Life Sciences, " -"Division of Genomics Technology, to expand this work on single cells " -"following a **population transcriptomics** approach ([Plessy and coll., 2013]" -"(https://pubmed.gov/23281054)) focused on sampling the largest possible " -"number of cells. In our ongoing developments, we have reached **single-cell " -"and single molecule resolution** through the introduction of transposase " -"fragmentation and unique molecular identifiers ([Poulain and coll., 2017]" -"(https://pubmed.gov/28349422)). The protocol exists in two versions, one for " -"FACS-isolated cells, and one for the Fluidigm C1 platform ([Kouno and coll., " -"2019](https://pubmed.gov/30664627))." +"In 2013–8, I lead a new development cycle at the Genomics Miniaturization " +"Technology Unit in RIKEN's Center for Life Sciences, Division of Genomics " +"Technology, to expand this work on single cells following a **population " +"transcriptomics** approach ([Plessy and coll., 2013](https://pubmed." +"gov/23281054)) focused on sampling the largest possible number of cells. In " +"our ongoing developments, we have reached **single-cell and single molecule " +"resolution** through the introduction of transposase fragmentation and " +"unique molecular identifiers ([Poulain and coll., 2017](https://pubmed." +"gov/28349422)). The protocol exists in two versions, one for FACS-isolated " +"cells, and one for the Fluidigm C1 platform ([Kouno and coll., 2019](https://" +"pubmed.gov/30664627))." msgstr "" #. type: Plain text @@ -96,7 +96,9 @@ msgid "" "(https://pubmed.gov/24904046)), and neurogenesis in the mouse olfactory " "epithelium using single-cell CAGE and ATAC-seq techniques. In parallel with " "this promoter-centric work, I have also explored the huge repertoire of the " -"**T cell antigen receptors**." +"T cell antigen receptors. I also applied the nanoCAGE technology to patient " +"samples infected with the human papillomavirus (HPV) ([Taguchi and coll., " +"2020](https://pubmed.gov/33093512))." msgstr "" #. type: Plain text