From: Charles <charles.plessy@oist.jp>
Date: Sun, 10 Mar 2019 01:44:06 +0000 (+0900)
Subject: CAPsmart
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=e0f351b904d3038731ea394b5716459185d6ebbd;p=source%2F.git

CAPsmart
---

diff --git a/biblio/25003736.mdwn b/biblio/25003736.mdwn
index 878bb0fe..6f726400 100644
--- a/biblio/25003736.mdwn
+++ b/biblio/25003736.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Four methods of preparing mRNA 5' end libraries using the illumina sequencing platform."]]
-[[!tag template_switching method oligo-capping benchmark]]
+[[!tag cap template_switching method oligo-capping benchmark]]
 
 Machida RJ, Lin YY.
 
diff --git a/tags/cap.mdwn b/tags/cap.mdwn
index 34d054e4..8e2ae7f3 100644
--- a/tags/cap.mdwn
+++ b/tags/cap.mdwn
@@ -56,6 +56,13 @@ in the [[template_switching]] tag page.
  - [[Kwak et al., 2013|biblio/23430654]] modified oligo-capping to create Pro-cap, a method for nuclear
    run-on analysis at single-nucleotide resulution.
 
+ - CapSMART ([[Machida and Lin, 2014|biblio/25003736]]): non-capped RNAs are
+   dephosphorylated, rephoshporylated and a blocking linker containing
+   isoguanosines and isocytidines (so that it can not be reverse-transcribed) is
+   ligated to them.  A classical SMART protocol follows.  cDNAs are amplified with
+   biotinylated forward primers, so that the 5′ end can be recovered after
+   mechanical fragmentation.
+
 Atypical caps (work in progress)
 --------------------------------