From: Charles Plessy <https://launchpad.net/~plessy>
Date: Sun, 26 Aug 2012 00:44:37 +0000 (+0900)
Subject: Un pour hier.
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=dd61d418756b02b3f7d61b609eea3e56eab1d020;p=source%2F.git

Un pour hier.
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+[[!meta title="Quantification of mRNA in single cells and modelling of RT-qPCR induced noise."]]
+[[!tag single_cell PCR noise reverse_transcription]]
+
+Bengtsson M, Hemberg M, Rorsman P, Ståhlberg A.
+
+BMC Mol Biol. 2008 Jul 17;9:63.
+
+Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.
+
+[[!pmid 18631407 desc="PCR noise is much smaller than biological noise for transcripts more abundant than 100 mRNA or 20 cDNA copies.  Reverse-transcription is possible in 40 mM guanidine thiocyanate.  Reported RT efficiencies vary between 99 and 2 %"]]