From: Charles Plessy Date: Mon, 16 Oct 2017 01:49:45 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=dcd41d76ac87849bc8522aed22b69850e99920ab;p=source.git Old --- diff --git a/biblio/15247328.mdwn b/biblio/15247328.mdwn new file mode 100644 index 00000000..2ba39417 --- /dev/null +++ b/biblio/15247328.mdwn @@ -0,0 +1,10 @@ +[[!meta title="In vitro selection of restriction endonucleases by in vitro compartmentalization."]] +[[!tag emulsion selection enzyme]] + +Nucleic Acids Res. 2004 Jul 6;32(12):e95 doi:10.1093/nar/gnh096 + +Doi N, Kumadaki S, Oishi Y, Matsumura N, Yanagawa H. + +In vitro selection of restriction endonucleases by in vitro compartmentalization. + +[[!pmid 15247328 desc="The cleaved DNA molecules are selected by filling of their ends with biotin-dUTP"]] diff --git a/biblio/To_Do b/biblio/To_Do index 4d867e21..5e926ce8 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -604,9 +604,6 @@ Oil droplets containing water droplets can be FACS sorted. 14500846 [emulsion] Improvement of STABLE by addtion of one biotinylated end, usage of wheat germ extract for translation, and reduction of the GC content of the streptavidin gene to facilitate PCR. -15247328 [emulsion] -The cleaved DNA molecules are selected by filling of their ends with biotin-dUTP - 15156154 [emulsion] Polymerases selected on the ability to amplify their gene despite a 3' mismach in the primers were shown to be able to incorporate a broad range of nucleotide analogs.