From: Charles Date: Sat, 13 Oct 2018 22:54:41 +0000 (+0900) Subject: Syntaxx X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=db4bd47ce2d11f08284ca727559d4afb8d5af76b;p=source.git Syntaxx --- diff --git a/biblio/10.1101_409086.mdwn b/biblio/10.1101_409086.mdwn index afb8cfe7..fa04afd8 100644 --- a/biblio/10.1101_409086.mdwn +++ b/biblio/10.1101_409086.mdwn @@ -7,4 +7,4 @@ bioRxiv, September 6, 2018. Xdrop: targeted sequencing of long DNA molecules from low input samples using droplet sorting -[[!DOI: 10.1101/409086 desc="Enrichment by 1) encapsulating DNA fragments in double-emulsion water-in-oil-in-water droplets, 2) sorting the droplets containing the target sequence detected by a hydrolysis probe PCR, 3) extracting DNA from the droplets, 4) amplifying it by MDA (in a second generation of droplets), and finally 5) sequencing. When enriching for HPV DNA in HeLa genomes, 5 to 10% of the reads were on target. Emulsions were prepared in Dolomite chips."]] +[[!doi 10.1101/409086 desc="Enrichment by 1) encapsulating DNA fragments in double-emulsion water-in-oil-in-water droplets, 2) sorting the droplets containing the target sequence detected by a hydrolysis probe PCR, 3) extracting DNA from the droplets, 4) amplifying it by MDA (in a second generation of droplets), and finally 5) sequencing. When enriching for HPV DNA in HeLa genomes, 5 to 10% of the reads were on target. Emulsions were prepared in Dolomite chips."]]