From: Charles Date: Tue, 4 Feb 2020 06:11:36 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=dab30558fe64feeb10dffab51e6c499dcee6dac6;p=source.git Café --- diff --git a/biblio/31719208.mdwn b/biblio/31719208.mdwn new file mode 100644 index 00000000..00673cc8 --- /dev/null +++ b/biblio/31719208.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Definitive demonstration by synthesis of genome annotation completeness."]] +[[!tag synthethic]] + +Jaschke PR, Dotson GA, Hung KS, Liu D, Endy D. + +Proc Natl Acad Sci U S A. 2019 Nov 26;116(48):24206-24213. doi:10.1073/pnas.1905990116 + +Definitive demonstration by synthesis of genome annotation completeness. + +[[!pmid 31719208 desc="A øX174 genome where most cryptic ORFs were erased is still viable. In its initial version, its growth was slower than the wild type, but an in vitro selection isolated a point mutant that recovered the growth phenotype. Additional experiments suggest that the point mutation affected the translation levels of one of the major ORFs. A øX174 genome where the major ORFs are mutated is not viable, and only a single cryptic ORF shows detectable levels of expression."]]