From: Charles Plessy Date: Thu, 19 Apr 2018 04:49:46 +0000 (+0900) Subject: CapSelect. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=da3664270d3e634ab6b9d47eb63be1e1a7b2ec96;p=source%2F.git CapSelect. --- diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn index f24e5178..4619e756 100644 --- a/tags/template_switching.mdwn +++ b/tags/template_switching.mdwn @@ -2,6 +2,11 @@ (work in progress) + - In the "_CapSelect_" method, [[Schmidt and Mueller, 1999|biblio/10518626]] + stimulate template switching with manganese (see below), tail the first-strand + cDNAs with dA, and add 5′ linkers with T4 DNA ligase and duplex adapters + ending with a (T)TTTGGG overhang. + - The "_terminal continuation_" method ([[Ginsberg et al., 2002|biblio/12462399]], [[Che et al., 2004|biblio/14647400]]) is essentially a template switching with DNA oligonucleotides ending in `CCC` or `GGG`. `AAA`