From: Charles Plessy Date: Mon, 5 Feb 2018 05:25:02 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=da2c05459682786d2a590567bc67569db45cb66b;p=source%2F.git Old --- diff --git a/biblio/7082652.mdwn b/biblio/7082652.mdwn new file mode 100644 index 00000000..f9e911d4 --- /dev/null +++ b/biblio/7082652.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Reversal of T4 RNA ligase."]] +[[!tag ligase]] + +Krug M, Uhlenbeck OC. + +Biochemistry. 1982 Apr 13;21(8):1858-64. + +Reversal of T4 RNA ligase. + +[[!pmid 7082652 desc="T4RNL1 & AMP can remove or exchange 3′ phosphate from RNA molecles. Thus, 3′-P is not a good blocking group. "]] diff --git a/biblio/To_Do b/biblio/To_Do index ed99b9da..d73e64a9 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -38,9 +38,6 @@ Uses T3N9 instead of T7dT during all the RT reactions. No 3' bias. Efficient on 14606961 [libraries] The nuclease activity of T7 RNA pol could also show some transcript specificity. -3799962 [enzymes] -Optimal : Donor/acceptor 5:1; hexammine cobalt chloride 5.0 mM, PEG8000 25%; ATP 100µM. Inhibitors: ATP > 100 µM, MN 2+ > 5mM, NaCl > 20 mM. - 14513556 [libraries] Hybridises single-stranded tester plasmid and driver PCR product, then purified single strand molecules out of duplexes using hydroxyapatite. @@ -350,9 +347,6 @@ The ultraconserved elements in the Irx clusters are transcription enhancers. 15899965 [enhancers] Out of 126 ultraconserved elements in a melanogaster / A. Gambiae comparison, only 1 is intergenic, the other being mostly ncRNA and exons. -7082652 [enzymes] -T4RNL1 & AMP can remove or exchange 3' pNp from RNA molecles. - 12429865 [tags] Provides an easy access to various statistical tests. diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn index afc1dd15..015ca741 100644 --- a/tags/ligase.mdwn +++ b/tags/ligase.mdwn @@ -13,7 +13,7 @@ On T4 RNL1: Adenylation efficiency after 6h: C >> U ~ A > G ([[McLaughlin et al., 1985|biblio/3978074]]). - Also used for single-strand DNA ligation ([[Tessier, Brousserau & Vernet, 1986|biblio/3799962]]) with PEG and hexamine cobalt chloride (HCC) as additives. - + - Can dephoshporylate RNA 3′ ends ([[Krug & Uhlenbeck, 1982|biblio/7082652]]). On T4 RNL2: