From: Charles Plessy <plessy@riken.jp>
Date: Thu, 17 May 2018 04:08:02 +0000 (+0900)
Subject: Onigiri.
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=d855c851f337bebf22521ece65b220bca436c2a5;p=source%2F.git

Onigiri.
---

diff --git a/biblio/28747695.mdwn b/biblio/28747695.mdwn
new file mode 100644
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+++ b/biblio/28747695.mdwn
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+[[!meta title="Compounds that enhance the tailing activity of Moloney murine leukemia virus reverse transcriptase."]]
+[[!tag reverse_transcription]]
+
+Sci Rep. 2017 Jul 26;7(1):6520. doi:10.1038/s41598-017-04765-8
+
+Ohtsubo Y, Nagata Y, Tsuda M.
+
+Compounds that enhance the tailing activity of Moloney murine leukemia virus reverse transcriptase.
+
+[[!pmid 28747695 desc="rA, r/dG, r/dC are potent enhancers of tailing.  In longer reactions, GMP, GDP or CDP promote continuous extension of the tail.  Reactions performed with 100 fmols of substrate DNA, 50 mM Tris-HCl pH 8.3, 75 mM KCl, 6 mM MgCl2, 2 mM DTT, 4 mM dATP, dCTP, dGTP, or dTTP, 4 mM MnCl2, and 50 U MMLV-RT, at 30 °C."]]
diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 6866b55e..8719370c 100644
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -36,10 +36,15 @@ transcriptases have a TdT activity.
    not available.)
 
  - Following an initial observation of [[Clark et al (1987)|biblio/3323527]] on
-   the Klenow fragment, [[Ohtsubo et al, 2017|biblio/28150748]] showed that
+   the Klenow fragment, [[Ohtsubo et al, 2017a|biblio/28150748]] showed that
    specific tailing is enhanced by the complementary dNMP (C enhanced by dGMP,
    etc.), except for A-tailing, which is already the strongest.
 
+ - Other nucleotides than dNMPs can enhance tailing.  In particular, rA, dA, dG
+   and dC potently induce tailing, and GMP, GDP and CDP induce continuous
+   tailing when longer reaction times are allowed ([[Ohtsubo et al.,
+   2017b|biblio/28747695]]).
+
 ### Reverse-transcriptases tolerate terminal mismatches
 
  - Reported by [[Mizuno et al., 1999|biblio/9973624]].