From: Charles Plessy <charles.plessy@oist.jp>
Date: Thu, 15 Dec 2022 04:39:53 +0000 (+0900)
Subject: 18S primers
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=d850dc33fc0236e9cbdcd77a2388f6f9d6a5a432;p=source--%2F.git

18S primers
---

diff --git a/biblio/19633714.mdwn b/biblio/19633714.mdwn
new file mode 100644
index 00000000..38e42bad
--- /dev/null
+++ b/biblio/19633714.mdwn
@@ -0,0 +1,17 @@
+[[!meta title="A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes."]]
+[[!tag eDNA]]
+
+Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM.
+
+PLoS One. 2009 Jul 27;4(7):e6372. doi:10.1371/journal.pone.0006372
+
+A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes.
+
+[[!pmid 19633714 desc="Primers contain degenerate sequences and are either universal or eukaryote-based.  Samples from Mount Hope Bay, Massachusetts and Palmer Station, Antarctica."]]
+
+    >18S_1380F eukaryotic 10.1371/journal.pone.0006372
+    CCCTGCCHTTTGTACACAC
+    >18S_1389F universal 10.1371/journal.pone.0006372
+    TTGTACACACCGCCC
+    >18S_1510R eukaryotic 10.1371/journal.pone.0006372
+    CCTTCYGCAGGTTCACCTAC
diff --git a/tags/eDNA.mdwn b/tags/eDNA.mdwn
index 48987409..f15996b4 100644
--- a/tags/eDNA.mdwn
+++ b/tags/eDNA.mdwn
@@ -18,6 +18,12 @@
 GCCAGTAGTCATATGCTTGTCT
 >18S_701R 10.1371/journal.pone.0073935
 GGAGCTGGAATTACCGC
+>18S_1380F eukaryotic 10.1371/journal.pone.0006372
+CCCTGCCHTTTGTACACAC
+>18S_1389F universal 10.1371/journal.pone.0006372
+TTGTACACACCGCCC
+>18S_1510R eukaryotic 10.1371/journal.pone.0006372
+CCTTCYGCAGGTTCACCTAC
 ```
 https://doi.org/10.1371/journal.pone.0073935