From: Charles Plessy <plessy@riken.jp>
Date: Mon, 21 May 2018 07:46:55 +0000 (+0900)
Subject: Old
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=d0ebac749428a68e8f6bfac7dc93d2d52c76e534;p=source.git

Old
---

diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn
index 5c3a1090..b78625de 100644
--- a/tags/template_switching.mdwn
+++ b/tags/template_switching.mdwn
@@ -45,7 +45,9 @@ as a replacement for RNA.
    RRR and RRL, using a 5′-focused method similar to nanoCAGE or STRT.
 
  - 3′ phosphate or biotin blocking groups abolish template-switching
-   ([[Turchinovich et al (2014)|biblio/24922482]] and others).
+   ([[Turchinovich et al (2014)|biblio/24922482]] and others).  However,
+   [[Pinto & Lindblad (2010)|biblio/19837043]] report the use of a
+   3′ C3 spacer (on all-DNA TSOs).
 
  - 5′ iso-dC and iso-dG prevents reverse-transcriptase to reach the end
    of the TSO, and therefore blocks concatenation
@@ -62,10 +64,12 @@ as a replacement for RNA.
    magnesium concentration (to 6 mM) or adding manganese at the end of the
    reaction (1 or 2 mM) increased the frequency of dC addition (moderately
    for Mg<sup>2+</sup> and strongly for Mn<sup>2+</sup>).  Enzyme: SSII; dNTP
-   concentration: 1 mM each.
+   concentration: 1 mM each.  [[Pinto & Lindblad (2010)|biblio/19837043]] also
+   used manganese.
 
  - [[Lee et al (2017)|biblio/28327113]] increased the efficiency of template
    switching non-capped molecules by increasing dNTPs to 2 mM and
    Mg<sup>2+</sup> to 9 mM.
 
+
 [[!inline pages="tagged(template_switching)" limit=0]]