From: Charles Plessy Date: Fri, 13 Oct 2017 13:08:09 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=d0d704cfbe6b4578b429137281aacbd5845bb0f0;p=source%2F.git Old --- diff --git a/biblio/15522920.mdwn b/biblio/15522920.mdwn new file mode 100644 index 00000000..8e2c16d2 --- /dev/null +++ b/biblio/15522920.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Covalent DNA display as a novel tool for directed evolution of proteins in vitro."]] +[[!tag emulsion selection]] + +Protein Eng Des Sel. 2004 Sep;17(9):699-707. Epub 2004 Nov 2. + +Bertschinger J, Neri D. + +Covalent DNA display as a novel tool for directed evolution of proteins in vitro. + +[[!pmid 15522920 desc="A methyl transferase fusion protein which binds covalently 5-fluorodesoxycytosine, gets linked to the constructs in which it is encoded."]] diff --git a/biblio/To_Do b/biblio/To_Do index f0aa4da6..4d867e21 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -580,9 +580,6 @@ Only the constructs encoding methylases escape degradation. 10471784 [emulsion] The gene product, fused to streptavidin, is bound to a biotinylated molecule in which it is encoded. -15522920 [emulsion] -A methyl transferase fusion protein which binds covalently 5-fluorodesoxycytosine, gets linked to the constructs in which it is encoded. - 12482612 [emulsion] As tyramide is converted to a free radical which reacts with neighbouring proteins, only beads displaying the epitope become fluorescent.