From: Charles Plessy <charles.plessy@oist.jp>
Date: Thu, 22 Dec 2022 07:24:08 +0000 (+0900)
Subject: en attendant
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=cfad6fe81448b8b6d9ca0564efd91d376298251f;p=source.git

en attendant
---

diff --git a/biblio/29506021.mdwn b/biblio/29506021.mdwn
new file mode 100644
index 00000000..e3cb72ad
--- /dev/null
+++ b/biblio/29506021.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Updating the 97% identity threshold for 16S ribosomal RNA OTUs."]]
+[[!tag eDNA]]
+
+Edgar RC.
+
+Bioinformatics. 2018 Jul 15;34(14):2371-2375. doi:10.1093/bioinformatics/bty113
+
+Updating the 97% identity threshold for 16S ribosomal RNA OTUs.
+
+[[!pmid 29506021 desc="Following a benchmark on V4 regions of the 16S rRNA, proposes to raise the threshold to 99% or to just use sequence variants (also called ZOTUS, Zero-radius OTUs."]]
diff --git a/tags/eDNA.mdwn b/tags/eDNA.mdwn
index f15996b4..10df54d7 100644
--- a/tags/eDNA.mdwn
+++ b/tags/eDNA.mdwn
@@ -11,6 +11,9 @@
 
  - A Nextflow pipeline: eDNAFlow [[Mousavi‐Derazmahalleh and coll., 2021|biblio/10.1111_1755-0998.13356]].
 
+ - Edgar ([[2018|biblio/29506021]] proposes to raise the threshold to 99% or to
+   just use sequence variants (also called ZOTUS, Zero-radius OTUs. 
+
 ### Published primers
 
 ```