From: Charles Date: Mon, 10 Feb 2020 04:34:58 +0000 (+0900) Subject: café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=cdf7888cc72bfc75ebd205d6687e0d59a1d251fc;p=source.git café --- diff --git a/biblio/23123427.mdwn b/biblio/23123427.mdwn new file mode 100644 index 00000000..a71b0591 --- /dev/null +++ b/biblio/23123427.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Simple and inexpensive three-step rapid amplification of cDNA 5' ends using 5' phosphorylated primers."]] +[[!tag reverse_transcription cap]] + +Dallmeier K, Neyts J. + +Anal Biochem. 2013 Mar 1;434(1):1-3. doi:10.1016/j.ab.2012.10.031 + +Simple and inexpensive three-step rapid amplification of cDNA 5' ends using 5' phosphorylated primers. + +[[!pmid 23123427 desc="No G-addition observed on mRNAs expressed from a SV40 promoter in Vero-B (African green monkey kidney) cells."]] diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index 8880db01..3b304dcd 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -102,6 +102,10 @@ nucleotide as a template. complex that suggests that a m7GpppNm / DNA duplex could form during the reverse-transcription of the cap + - Nevertheless, in [[Dallmeier and Neyts, 2013|biblio/23123427]], where + first-strand cDNAs prepared with a phosphorylated reverse-transcription + primer were circularised, amplified and sequenced, there is not visible + evidence for G addition. ### Reverse-transcriptases tolerate terminal mismatches