From: Charles Plessy Date: Tue, 7 Nov 2017 08:18:08 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=cc92996e41cb48c663d224f493e94bebddadcbcd;p=source%2F.git Old --- diff --git a/biblio/15205470.mdwn b/biblio/15205470.mdwn new file mode 100644 index 00000000..eae98df5 --- /dev/null +++ b/biblio/15205470.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes."]] +[[!tag ligation]] + +Nucleic Acids Res. 2004 Jun 17;32(11):e86 doi:10.1093/nar/gnh085 + +Cole K, Truong V, Barone D, McGall G. + +Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes. + +[[!pmid 15205470 desc="T4 RNA ligase reaction efficiency improved by PEG and pre-adenylation. Direct labelling method for big quantities: RNA fragmentation, then T4 RNA ligation of biotinylated oligos."]] diff --git a/biblio/To_Do b/biblio/To_Do index 9f82fcfd..de718c32 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -47,10 +47,6 @@ Single cell RT-PCR. 12711698 [amplification] [tracked] Uses T3N9 instead of T7dT during all the RT reactions. No 3' bias. Efficient on degraded RNA. Independant of polyadenylation (useful for non-polyA genes, like histones). -15205470 [libraries] [tracked] -T4 RNA ligase reaction efficiency improved by PEG and pre-adenylation. -direct labelling method for big quantities: RNA fragmentation, then T4 RNA ligation of biotinylated oligos. - 14606961 [libraries] The nuclease activity of T7 RNA pol could also show some transcript specificity.