From: Charles Plessy Date: Thu, 17 May 2018 06:52:40 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=cc52cf1291a9f0777d4682a131c1bfe468b85d2f;p=source.git Café --- diff --git a/biblio/20598146.mdwn b/biblio/20598146.mdwn new file mode 100644 index 00000000..9aa458fd --- /dev/null +++ b/biblio/20598146.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samples."]] +[[!tag template_switching reverse_transcription]] + +BMC Genomics. 2010 Jul 2;11:413. doi:10.1186/1471-2164-11-413 + +Kapteyn J, He R, McDowell ET, Gang DR. + +Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samples. + +[[!pmid 20598146 desc="TSO starting with iCiGiC (iso-dC / iso-dG) does not form concatenates because the RTase is inhibited by these nucleotides, therefore it does not reach the end and no extra template switching occurs."]] diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn index e7f8577a..5c3a1090 100644 --- a/tags/template_switching.mdwn +++ b/tags/template_switching.mdwn @@ -47,6 +47,10 @@ as a replacement for RNA. - 3′ phosphate or biotin blocking groups abolish template-switching ([[Turchinovich et al (2014)|biblio/24922482]] and others). + - 5′ iso-dC and iso-dG prevents reverse-transcriptase to reach the end + of the TSO, and therefore blocks concatenation + ([[Kapteyn et al., 2010|biblio/20598146]]). + ### Effect of TSO concentration - For the STRT method, [[Zajac et al (2013)|biblio/24392002]] concluded that