From: Charles Plessy <charles.plessy@oist.jp>
Date: Fri, 8 Feb 2019 07:31:20 +0000 (+0900)
Subject: Specificity of RT priming at higher temperatures.
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=ca577f93ca6412a06b1b1d33139e72313d906150;p=source.git

Specificity of RT priming at higher temperatures.
---

diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 9e31aa18..c73e930f 100644
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -89,6 +89,9 @@ nucleotide as a template.
  - Reported by [[Mizuno et al., 1999|biblio/9973624]].
  - Utilised in [[Arnaud et al., 2016|biblio/27071605]] to reduce priming or
    ribosomal or hemoglobin RNA.
+ - Specificity of RT nevertheless increases with reaction temperature.  For
+   instance, [[Xu and coll., 2019|biblio/30573814]] increased it from
+   ~30 % at 47 °C to ~75 % at 55 °C.
 
 ### Reverse-transcription primers