From: Charles Plessy Date: Wed, 9 Feb 2011 08:22:35 +0000 (+0900) Subject: # Template switching. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=c895e54c209f098d2ea80c57b127cc6664cd6691;p=source.git # Template switching. --- diff --git a/biblio/20876692.mdwn b/biblio/20876692.mdwn new file mode 100644 index 00000000..27bb1395 --- /dev/null +++ b/biblio/20876692.mdwn @@ -0,0 +1,3 @@ +[[!meta title="Reverse transcriptases can clamp together nucleic acids strands with two complementary bases at their 3'-termini for initiating DNA synthesis."]] +[[!tag template_switching enzyme manganese]] +[[!pmid 20876692 desc="AT-rich cDNA tails are not triggering template-switching well. CC are the strongest. Mn₂⁺ potentiates template switching for MMLV. dGTP stabilises the clamp between the enzyme and the nucleic acids."]]