From: Charles Plessy Date: Wed, 20 Sep 2017 00:06:52 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=c6e11eee2ef27eee7617b84e04ee6f13c5d4d04f;p=source%2F.git Old --- diff --git a/biblio/15642699.mdwn b/biblio/15642699.mdwn new file mode 100644 index 00000000..73542b09 --- /dev/null +++ b/biblio/15642699.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Isolation and characterization of a thermostable RNA ligase 1 from a Thermus scotoductus bacteriophage TS2126 with good single-stranded DNA ligation properties."]] +[[!tag enzyme ligase]] + +Nucleic Acids Res. 2005 Jan 7;33(1):135-42. doi:10.1093/nar/gki149 + +Blondal T, Thorisdottir A, Unnsteinsdottir U, Hjorleifsdottir S, Aevarsson A, Ernstsson S, Fridjonsson OH, Skirnisdottir S, Wheat JO, Hermannsdottir AG, Sigurdsson ST, Hreggvidsson GO, Smith AV, Kristjansson JK. + +Isolation and characterization of a thermostable RNA ligase 1 from a Thermus scotoductus bacteriophage TS2126 with good single-stranded DNA ligation properties. + +[[!pmid 15642699 desc="Preference for circularisation. Optimum : 65-70 °C, 25 µM ATP, pH 7.5-8, 10× more efficient than commercial ones."]] diff --git a/biblio/To_Do b/biblio/To_Do index 4a0fddb3..bf358a97 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -365,9 +365,6 @@ Presentation of the Ribo-SPIA kit, which can replace T7 in vitro translation for 7732590 [misc] [review] Reminds that in some organisms, most transcripts share their 5' end. -15642699 [enzymes] -Preference for circularisation. Optimum : 65-70ºC, 25µM ATP, pH 7.5-8, 10x more efficient than commercial ones. - 15590943 [genome] Distinguishes "stable" from "variable" deserts. Stable deserts contain more evolutionary conserved regions, compared to variable deserts.