From: Charles Plessy Date: Thu, 14 Oct 2021 07:38:45 +0000 (+0900) Subject: oburo X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=bf57dc996bdb841392a55351b9cc4bb7a2f70bae;p=source%2F.git oburo --- diff --git a/biblio/34255842.mdwn b/biblio/34255842.mdwn new file mode 100644 index 00000000..d7daba71 --- /dev/null +++ b/biblio/34255842.mdwn @@ -0,0 +1,10 @@ +[[!meta title="capCLIP: a new tool to probe translational control in human cells through capture and identification of the eIF4E-mRNA interactome."]] +[[!tag cap method library]] + +Jensen KB, Dredge BK, Toubia J, Jin X, Iadevaia V, Goodall GJ, Proud CG. + +Nucleic Acids Res. 2021 Oct 11;49(18):e105. doi:10.1093/nar/gkab604 + +capCLIP: a new tool to probe translational control in human cells through capture and identification of the eIF4E-mRNA interactome. + +[[!pmid 34255842 desc="eIF4E flagged via CRISPR/Cas9 gene editing. mRNAs were crosslinked to eIF4E and immunoprecipitated with anti-flag antibodies. First-strand cDNAs primed via a linker ligated in 3′. This method (capCLIP) was used to study the effect of rapamycin treatment and eIF4E phosphorylation."]] diff --git a/tags/cap.mdwn b/tags/cap.mdwn index 552aa76d..175d7267 100644 --- a/tags/cap.mdwn +++ b/tags/cap.mdwn @@ -63,6 +63,11 @@ in the [[template_switching]] tag page. biotinylated forward primers, so that the 5′ end can be recovered after mechanical fragmentation. + - capCLIP ([[Jensen and coll., 2021|biblio/34255842]]): eIF4E is flagged by + gene editing, mRNA crosslinked to eIF4E and the whole is immunoprecipitated. + This circumvents the problem of access to good eIF4E antibodies for + immunoprecipitation. + Atypical caps (work in progress) --------------------------------