From: Charles Plessy Date: Fri, 13 Mar 2020 08:47:03 +0000 (+0900) Subject: RT with low G-addition. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=bdde91a25886e77169745016ee10ddac8002f5c9;p=source%2F.git RT with low G-addition. --- diff --git a/biblio/31363093.mdwn b/biblio/31363093.mdwn new file mode 100644 index 00000000..0f92090a --- /dev/null +++ b/biblio/31363093.mdwn @@ -0,0 +1,12 @@ +[[!meta title="MAPCap allows high-resolution detection and differential expression analysis of transcription start sites."]] +[[!tag method library reverse-transcription]] + +Bhardwaj V, Semplicio G, Erdogdu NU, Manke T, Akhtar A. + +Nat Commun. 2019 Jul 30;10(1):3219. doi:10.1038/s41467-019-11115-x + +MAPCap allows high-resolution detection and differential expression analysis of transcription start sites. + +[[!pmid 31363093 desc="Reports that in their reverse-transcription reaction, G-addition is very low."]] + +RT reaction in 20 µL: 10x Buffer 2 μL; dNTP (10 mM) 1 μL → 500 nM final; MgCl2 (25 mM) 4 μL → 5 mM final; DTT (0.1 M) 2 μL → 10 mM final; RNaseOUT (40 U/μl) 1 μL; SSIII (200 U/μL) 1 μL diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index 3555b57d..618d0eca 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -65,6 +65,8 @@ to mismatches can be the explanation. - [[Mohr and coll, 2013|biblio/23697550]] showed that the Thermostable group II intron reverse transcriptase also adds non-templated As. + - [[Bhardwaj and coll, 2020|biblio/31363093]] report a reverse-transcription + with very low G-addition. ### Templated TdT activity