From: Charles Date: Fri, 8 Mar 2019 05:13:55 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=bcd59f3d8dc92275f591555bc7f9f6e1ba3b69d0;p=source%2F.git Café --- diff --git a/biblio/3028775.mdwn b/biblio/3028775.mdwn new file mode 100644 index 00000000..2962fcd2 --- /dev/null +++ b/biblio/3028775.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Cloning of the human cDNA for the U1 RNA-associated 70K protein."]] +[[!tag cap method library]] + +Theissen H, Etzerodt M, Reuter R, Schneider C, Lottspeich F, Argos P, Lührmann R, Philipson L. + +EMBO J. 1986 Dec 1;5(12):3209-17. + +Cloning of the human cDNA for the U1 RNA-associated 70K protein. + +[[!pmid 3028775 desc="Clones the full-length cDNA by affinity purification with m7G antibodies, and RNAse A digestion of the incomplete cDNA/RNA duplexes. Cites Schneider 1986 for the method, but could not find this reference."]] diff --git a/tags/cap.mdwn b/tags/cap.mdwn index afc78ea9..351bb60b 100644 --- a/tags/cap.mdwn +++ b/tags/cap.mdwn @@ -10,6 +10,11 @@ Methods for enriching capped RNAs - See the Wikipedia for [[CAGE methods|https://en.wikipedia.org/wiki/Cap_analysis_gene_expression]] (Cap Analysis Gene Expression). + - [[Thiessen and coll., 1986|biblio/3028775]] cloned the full-length cDNA by + affinity purification with m7G antibodies, and RNAse A digestion of the + incomplete cDNA/RNA duplexes. They cite Schneider 1986 for the method, but + I could not find this reference. + - A method similar to oligo-capping was reported by [[Sekine and Kato|biblio/8247743]] in 1993. - Oligo-capping ([[Maruyama et al., 1994|biblio/8125298]]): dephosphorylate,