From: Charles Date: Thu, 23 Jan 2020 09:22:35 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=bbd169af4ea8396931cdd7b2856d51aa29805279;p=source.git Café --- diff --git a/biblio/10.1101_2020.01.16.905182.mdwn b/biblio/10.1101_2020.01.16.905182.mdwn new file mode 100644 index 00000000..a19df767 --- /dev/null +++ b/biblio/10.1101_2020.01.16.905182.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Simple and efficient measurement of transcription initiation and transcript levels with STRIPE-seq"]] +[[!tag bioRxiv promoter method]] + +bioRxiv Posted January 16, 2020 doi:10.1101/2020.01.16.905182 + +Robert A. Policastro, R. Taylor Raborn, Volker P. Brendel and Gabriel E. Zentner + +Simple and efficient measurement of transcription initiation and transcript levels with STRIPE-seq + +[[!doi 10.1101/2020.01.16.905182 desc="Terminator-treated RNA reverse-transcribed with random N5 primers. The (biotinylated) TSO is added in the last 5 min of the RT (~40 µM final). The TSO carries a P5 linker and the RTP a P7 (indexed) linker. Follows a standard PCR and sequencing. The YR motif is detected in yeast and K562 cells. High amounts of rRNA remains in yeast"]]