From: Charles Plessy Date: Thu, 15 Feb 2018 04:01:50 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=b324eb2b2a33993d566020f548787008b022e84a;p=source.git Old --- diff --git a/biblio/16540077.mdwn b/biblio/16540077.mdwn new file mode 100644 index 00000000..f88efdcb --- /dev/null +++ b/biblio/16540077.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Direct amplification of single-stranded DNA for pyrosequencing using linear-after-the-exponential (LATE)-PCR."]] +[[!tag amplification PCR]] + +Salk JJ, Sanchez JA, Pierce KE, Rice JE, Soares KC, Wangh LJ. + +Anal Biochem. 2006 Jun 1;353(1):124-32 doi:10.1016/j.ab.2006.02.012 + +Direct amplification of single-stranded DNA for pyrosequencing using linear-after-the-exponential (LATE)-PCR. + +[[!pmid 16540077 desc="The PPi produced during the PCR can be removed with the pyrosequencing enzymes themselves. The antarctic phosphatase from NEB was not very efficient at this task."]] diff --git a/biblio/To_Do b/biblio/To_Do index af4f14a6..7f3abe58 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -467,9 +467,6 @@ Not read. Enhancers of Krox-20, conserved between chicken and mouse. 16527264 [enhancers] Not read. An enhancer of Rpx/Hesx1 conserved between mouse and xenopus. -16540077 [misc] -The PPi produced during the PCR can be removed with the pyrosequencing enzymes themselves. The antarctic phosphatase from NEB was not very efficient at this task. - 9380524 [protocols] Concentrations as high as 1 M or 2.5 M can strongly improve the yield.