From: Charles Plessy Date: Sat, 23 Dec 2023 06:13:20 +0000 (+0900) Subject: Dans l'avion. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=af48912ff1a9d749ac1ef031cd0e2c2b9e2a05e9;p=source--%2F.git Dans l'avion. --- diff --git a/biblio/38130951.mdwn b/biblio/38130951.mdwn new file mode 100644 index 00000000..aa2e426b --- /dev/null +++ b/biblio/38130951.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Functional specialization of Aurora kinase homologs during oogenic meiosis in the tunicate Oikopleura dioica."]] +[[!tag Oikopleura H3S28p H3S10p]] + +Feng H, Thompson EM. + +Front Cell Dev Biol. 2023 Dec 7;11:1323378. doi:10.3389/fcell.2023.1323378 + +Functional specialization of Aurora kinase homologs during oogenic meiosis in the tunicate Oikopleura dioica. + +[[!pmid 38130951 desc="“a C-terminal GFP fusion [of OdAur1] was localized on centrosomes throughout mitosis” “a commercial phospho-Aurora antibody that can recognize the phosphorylated forms of both Aurora1 and Aurora2 in O. dioica [localised] on centrosomes and chromosomes in prophase and metaphase, and on centrosomes and the central spindle in anaphase” “This suggests that Aurora1 and Aurora2 in O. dioica represent the polar and equatorial forms of Aurora kinases”. “[Knockdown] suggests that Aurora1 is involved in promoting the progression of prometaphase I. In all the Aur2 KD oocytes, both H3-pS10 and H3-pS28 were absent, and chromosomes were decondensed, reminiscent of INCENPa knockdown phenotypes”"]] diff --git a/tags/H3S28p.mdwn b/tags/H3S28p.mdwn index 1963c8f2..c1ef8182 100644 --- a/tags/H3S28p.mdwn +++ b/tags/H3S28p.mdwn @@ -47,4 +47,8 @@ The rat monoclonal antibody (Abcam ab10543) stains the centromere-attracting body in _O. dioica_, Osaka lab. strain. ([[Nishida and coll., 2021|biblio/34755656]]), probably by cross-reactivity. +In [[Feng and Thompson, 2023|biblio/38130951], the Abcam ab10543 antibody “was +either spread along entire chromosomes in 38% of oocytes or enriched on +centromeres in 62% of oocytes”. + [[!inline pages="tagged(H3S28p)" limit=0]] diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn index d95bb1a3..3c2a35be 100644 --- a/tags/Oikopleura.mdwn +++ b/tags/Oikopleura.mdwn @@ -261,6 +261,9 @@ Genes and pathways - 6 FGF genes were detected by Oulion, Bertrand and Escriva ([[2012|biblio/22919541]]). - _Eya_, _Pitx_, _Six1/2_, _Six3/6a_ and _Six3/6b_, with expression patterns supporting homology of larvacean and vertebrate placodes ([[Bassham and Postlethwait, 2005|biblio/16120641]]). + - There are two copies of Aurora, _OdAur1_ and _OdAur2_. In meiosis, they + localise to the centrosomes and the spindle respectively ([[Feng and + Thompson, 2023|biblio/38130951]]). ### Lost