From: Charles Plessy <plessy@riken.jp>
Date: Fri, 20 Oct 2017 00:49:08 +0000 (+0900)
Subject: Old
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=ac8fd318bd74e59d2addc566ff4952ebfefabc41;p=source.git

Old
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diff --git a/biblio/16461948.mdwn b/biblio/16461948.mdwn
new file mode 100644
index 00000000..91c86d6b
--- /dev/null
+++ b/biblio/16461948.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Optimization of in vitro transcription and full-length cDNA synthesis using the T4 bacteriophage gene 32 protein."]]
+[[!tag reverse_transcription]]
+
+J Biomol Tech. 2005 Sep;16(3):239-47
+
+Piché C, Schernthaner JP.
+
+Optimization of in vitro transcription and full-length cDNA synthesis using the T4 bacteriophage gene 32 protein.
+
+[[!pmid 16461948 desc="T4gp32 increases the yield of the smallest cDNAs by 10 %."]]
diff --git a/biblio/To_Do b/biblio/To_Do
index 833287f0..aaca0a6b 100644
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -661,9 +661,6 @@ Not read. An enhancer of Rpx/Hesx1 conserved between mouse and xenopus.
 16540077 [misc]
 The PPi produced during the PCR can be removed with the pyrosequencing enzymes themselves. The antarctic phosphatase from NEB was not very efficient at this task.
 
-16461948 [protocols]
-T4gp32 increases the yield of the smallest cDNAs by 10 %.
-
 2326177 [protocols]
 More inert than glycogen.
 
diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 04109217..2f8af9a5 100644
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -2,6 +2,10 @@
 
 (redaction in progress)
 
+Additives that increase reaction performance:
+
+ - T4 bacteriophage gene 32 protein (T4gp32, [[Piché _et al._, 2005|biblio/16461948]]).
+
 Reverse-transcriptases have a DNA-dependent DNA polymerase activity.
  - It is utilised in [[template_switching]] methods to add linkers to first-strand cDNAs.
  - It is also a source of antisense artefacts when the RT makes a second-strand cDNA