From: Charles <charles.plessy@oist.jp>
Date: Thu, 16 Jul 2020 04:01:25 +0000 (+0900)
Subject: Café
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=a8d691e143ee5aeb42debba8df002b4ed52032e2;p=source.git

Café
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+[[!meta title=""]]
+[[!tag sequence_tag bias]]
+
+Measuring sequencer size bias using REcount: a novel method for highly accurate Illumina sequencing-based quantification.
+
+Gohl DM, Magli A, Garbe J, Becker A, Johnson DM, Anderson S, Auch B, Billstein B, Froehling E, McDevitt SL, Beckman KB.
+
+Genome Biol. 2019 Apr 29;20(1):85. doi:10.1186/s13059-019-1691-6
+
+[[!pmid 31036053 desc="Sequencing templates already containing full Illumina adapters are cleaved from plasmids with a type IIS enzyme that generates blunt ends.  Comparison between fixed-length and variable-length payloads allowed to study the size bias in Illumina sequencers.  NextSeq > iSeq > MiSeq > NovaSeq.  A freeze-thaw cycle reduced the prevalence of the smallest templates (~150 bp)."]]