From: Charles Plessy Date: Fri, 28 Feb 2020 05:06:05 +0000 (+0900) Subject: Typo X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=a5c48bb760ca2a128efe83997be9593b348ce355;p=source%2F.git Typo --- diff --git a/biblio/7511410.mdwn b/biblio/7511410.mdwn index df2599b9..9ce07cb8 100644 --- a/biblio/7511410.mdwn +++ b/biblio/7511410.mdwn @@ -7,4 +7,4 @@ Biochemistry. 1994 Apr 5;33(13):3890-5 doi:10.1021/bi00179a014 Fidelity of in vitro DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase. -[[!pmid 7511410 desc="When the extra base added by the RTase in a template-switching reaction is determined by sequencing, the result does not reflect previous results obrained by primer extension assay (where A >> C on non-capped blunt DNA/RNA ends). This may be because of the differential efficiency of the RTase to extend a template over various single-base mismatches. In this assay, T to C and G to C were more frequent than T to A and G to A. Nevertheless, the experiments support previous evidence that addition is mostly limited to a single nucleotide. RT reaction: 50 mM Tris-HCl, pH 8.0; 75 mM KCl;, 0.1 mM EDTA; 1 mM DTT; 0.1% Triton X-100; 100 µM each dNTP; 7 mM MgCl2; 200 nM 24-base DNA-40-base RNA primer-template; 700 nM 41-base RNA template 2; 700 nM 42-base RNA template 3; and 100 nM HIV-1 RT in a final volume of 10 µL. When reaction products were to be sequenced, mixtures were incubated at 37 °C for 2 h."]] +[[!pmid 7511410 desc="When the extra base added by the RTase in a template-switching reaction is determined by sequencing, the result does not reflect previous results obtained by primer extension assay (where A >> C on non-capped blunt DNA/RNA ends). This may be because of the differential efficiency of the RTase to extend a template over various single-base mismatches. In this assay, T to C and G to C were more frequent than T to A and G to A. Nevertheless, the experiments support previous evidence that addition is mostly limited to a single nucleotide. RT reaction: 50 mM Tris-HCl, pH 8.0; 75 mM KCl;, 0.1 mM EDTA; 1 mM DTT; 0.1% Triton X-100; 100 µM each dNTP; 7 mM MgCl2; 200 nM 24-base DNA-40-base RNA primer-template; 700 nM 41-base RNA template 2; 700 nM 42-base RNA template 3; and 100 nM HIV-1 RT in a final volume of 10 µL. When reaction products were to be sequenced, mixtures were incubated at 37 °C for 2 h."]]