From: Charles Date: Mon, 6 Jul 2020 05:00:38 +0000 (+0900) Subject: Cleanup X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=a0bb5370bcb96c02b561cdaac07c5f519b31e97b;p=setup%2F.git Cleanup --- diff --git a/biblio/16790564.mdwn b/biblio/16790564.mdwn new file mode 100644 index 00000000..cb8160df --- /dev/null +++ b/biblio/16790564.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Primer Extension Enrichment Reaction (PEER): A New Subtraction Method for Identification of Genetic Differences Between Biological Specimens"]] +[[!tag method protocol]] + +Ganova-Raeva L, Zhang X, Cao F, Fields H, Khudyakov Y. + +Nucleic Acids Res. 2006 Jun 21;34(11):e76. doi: 10.1093/nar/gkl391 + +Primer Extension Enrichment Reaction (PEER): A New Subtraction Method for Identification of Genetic Differences Between Biological Specimens + +[[!pmid 16790564 desc="Generates primers by MmeI cleavage of cDNA extremities, and inactivates those which are complementary to the driver library by extending them with ddNTP"]] diff --git a/biblio/To_Do b/biblio/To_Do index 8021e42b..99956f1d 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -512,9 +512,6 @@ rasiRNA, the drosophila piRNA, are 5'P and one of their 2' or 3' is not OH. They 16778056 [small RNAs] Its neighbors got pseudogenized too in humans. -16790564 [protocols] -Generates primers by MmeI cleavage of cDNA extremities, and inactivates those which are complementary to the driver library by extending them with ddNTP. - 16822854 [protocols] The fluorophore and the quencher are separated by the strand-displacement activity of the enzyme. @@ -833,9 +830,6 @@ Evidence for complex polyA minus mRNA. 18836037 [misc] [not read] Having a binding site is not always essential. -18829718 [enzymes] -[not read]De-adenylates at 35 ℃, and circularises at 65 ℃. - 18988746 [genome] [not read] Many chromosomal aberrations in ES cells.