From: Charles Plessy Date: Fri, 13 Oct 2017 11:26:00 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=9ea9fa66c6cc7bc19817693ddcf06ed1aa489b8d;p=source.git Old --- diff --git a/biblio/15644201.mdwn b/biblio/15644201.mdwn new file mode 100644 index 00000000..644f9d45 --- /dev/null +++ b/biblio/15644201.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery."]] +[[!tag emulsion selection]] + +J Mol Biol. 2005 Feb 4;345(5):1015-26. doi:10.1016/j.jmb.2004.11.017 + +Bernath K, Magdassi S, Tawfik DS. + +Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery. + +[[!pmid 15644201 desc="Selection pressure can be increased by reducing the volume of the spheres."]] diff --git a/biblio/To_Do b/biblio/To_Do index 4984443b..f0aa4da6 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -592,9 +592,6 @@ Random primers were used to introduce mutations. 11274352 [emulsion] No beads. A bacterial cel is used as a subcompartment maintaining gene and proteins together. Evidence for leakage of small molecules at high compartment density. -15644201 [emulsion] -Selection pressure can be increased by reducing the volume of the spheres. - 14990785 [emulsion] Abil EM90 oil is inert enough to allow protein experssion in the RRL system.