From: Charles Date: Fri, 25 Dec 2020 05:05:49 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=972f87da614c3b41b351d466e4bcf393af5c11f0;p=source.git Café --- diff --git a/biblio/10.1101_2020.12.23.423594.mdwn b/biblio/10.1101_2020.12.23.423594.mdwn new file mode 100644 index 00000000..3c2f8fbf --- /dev/null +++ b/biblio/10.1101_2020.12.23.423594.mdwn @@ -0,0 +1,10 @@ +[[!meta title="SLIDR and SLOPPR: Flexible identification of spliced leader trans-splicing and prediction of eukaryotic operons from RNA-Seq data"]] +[[!tag Oikopleura bioRxiv]] + +Marius Wenzel, Berndt Mueller, Jonathan Pettitt + +bioRxiv 2020.12.23.423594; doi:10.1101/2020.12.23.423594 + +SLIDR and SLOPPR: Flexible identification of spliced leader trans-splicing and prediction of eukaryotic operons from RNA-Seq data + +[[!doi 10.1101/2020.12.23.423594 desc="Median intercistronic distance of 33 nt in Oikopleura. Calculated as the the distance between two "gene" annotations."]] diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn index ab0d6eb5..7821f2c2 100644 --- a/tags/Oikopleura.mdwn +++ b/tags/Oikopleura.mdwn @@ -282,7 +282,8 @@ Transcriptome 40-nt 5′ [[splice leader|tags/trans-splicing]] (SL) bearing a trimethylated cap is found in some RNAs. The SL gene is found downstream of the 5S RNA gene, which is repeated multiple times in the genome. The 3′ acceptor site has a strong UUU(C/U/A)AG consensus. - Reported intercistronic regions are short (<30 nt) ([[Ganot et al., 2004|biblio/15314184]]). + Reported intercistronic regions are short: <30 nt ([[Ganot et al., 2004|biblio/15314184]]) + or ~33 nt ([[Wenzel, Mueller and Pettitt, 2020|biblio/10.1101_2020.12.23.423594]]). - The splice leader found in the Norwegian strain by ([[Ganot et al., 2004|biblio/15314184]]) was found indentical in a Japanese strain by ([[Wang and coll., 2015|biblio/26032664]]). - A study using CAGE found that 39% of annotated gene models are trans-spliced with the