From: Charles Plessy Date: Tue, 8 Jul 2014 11:03:52 +0000 (+0900) Subject: Lu au bureau. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=96c447e8fd1a511e2c0fb3e9f9edf6d27ab3e022;p=source.git Lu au bureau. --- diff --git a/biblio/2833725.mdwn b/biblio/2833725.mdwn new file mode 100644 index 00000000..f028e43f --- /dev/null +++ b/biblio/2833725.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Second-strand cDNA synthesis with E. coli DNA polymerase I and RNase H: the fate of information at the mRNA 5' terminus and the effect of E. coli DNA ligase."]] +[[!tag cDNA method]] + +D'Alessio JM, Gerard GF. + +Nucleic Acids Res. 1988 Mar 25;16(5):1999-2014. + +Second-strand cDNA synthesis with E. coli DNA polymerase I and RNase H: the fate of information at the mRNA 5' terminus and the effect of E. coli DNA ligase. + +[[!pmid 2833725 desc="RNAse H (also provided by the reverse-transcriptase) and ligase are not strictly necessary during second-strand synthesis."]]