From: Charles Plessy Date: Fri, 26 Dec 2025 04:59:09 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=963c95d17655c357e1fc0562847eeab982535e4f;p=source--%2F.git Café --- diff --git a/biblio/40773350.mdwn b/biblio/40773350.mdwn new file mode 100644 index 00000000..b1ad7671 --- /dev/null +++ b/biblio/40773350.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Protocol for the isolation, culture, and transfection of squid primary cells"]] +[[!tag cell_culture]] + +Kim Y, Tanner HM, Rosenthal JJC, Brangwynne CP + +STAR Protoc. 2025 Sep 19;6(3):103994. doi:10.1016/j.xpro.2025.103994 + +Protocol for the isolation, culture, and transfection of squid primary cells + +[[!pmid 40773350 desc="Fibroblast-like cells migrate out of optic lobe, eye or gill explants after trypsin digestion, and can be transfected, but no cell division was observed. Insulin-transferrin-selenium (ITS-G), FGF and EGF are present in the cell culture medium."]] diff --git a/tags/cell_culture.mdwn b/tags/cell_culture.mdwn index 4383346d..7ee49049 100644 --- a/tags/cell_culture.mdwn +++ b/tags/cell_culture.mdwn @@ -10,5 +10,6 @@ A few notes that just scratch the surface of a vast field… - [[Kawamura and coll. (2021)|biblio/33899125]] reported the use of plasmin to establish coral cell lines. - Shark cells need high osmolarity and a supplement of 333 mM urea, 188 mM NaCl and 54 mM trimethylamine N-oxide ([[Uno and coll., 2020|biblio/33159152]]). - RasV12 could immortalise _Drosophila_ cells ([[Simcox and coll. 2008|biblio/18670627]]). Such lines seem to preferentially originate from adult muscle precursor cells ([[Dequéant and coll., 2015|biblio/26438832]]). + - Primary squid cell culture from explants in ITS-G, FGF and EGF ([[Kai and coll., 2025|biblio/40773350]]). [[!inline pages="tagged(cell_culture)" limit=0]]