From: Charles Plessy Date: Tue, 6 Feb 2018 03:55:27 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=95754280c5ae7d773e3afdf09508a6be90c1d564;p=source.git Old --- diff --git a/biblio/12448877.mdwn b/biblio/12448877.mdwn new file mode 100644 index 00000000..d18baa86 --- /dev/null +++ b/biblio/12448877.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Global amplification of cDNA from limiting amounts of tissue. An improved method for gene cloning and analysis"]] +[[!tag RACE ligase amplification protocol]] + +Mol Biotechnol. 2002 Nov;22(3):223-30 doi:10.1385/MB:22:3:223 + +Reddy MK, Nair S, Sopory SK. + +Global amplification of cDNA from limiting amounts of tissue. An improved method for gene cloning and analysis. + +[[!pmid 12448877 desc="Ligating a DNA adaptor 3′ to the FScDNA using T4 RNA ligase."]] diff --git a/biblio/To_Do b/biblio/To_Do index c387d338..700fcf51 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -473,9 +473,6 @@ Not read. An enhancer of Rpx/Hesx1 conserved between mouse and xenopus. 16540077 [misc] The PPi produced during the PCR can be removed with the pyrosequencing enzymes themselves. The antarctic phosphatase from NEB was not very efficient at this task. -12448877 [protocols] -Ligating a DNA adaptor 3' to the FScDNA using T4RNA ligase. - 9380524 [protocols] Concentrations as high as 1 M or 2.5 M can strongly improve the yield. diff --git a/tags/RACE.mdwn b/tags/RACE.mdwn index ca36e4ac..702e78e6 100644 --- a/tags/RACE.mdwn +++ b/tags/RACE.mdwn @@ -1,4 +1,3 @@ [[!meta title="pages tagged RACE"]] -[[!inline pages="tagged(RACE)" actions="no" archive="yes" -feedshow=10]] +[[!inline pages="tagged(RACE)" limit=0]]