From: Charles Plessy Date: Wed, 20 Sep 2017 02:22:06 +0000 (+0900) Subject: Old. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=931dacddcab278d1525daffd6ed71c51b8e8eb73;p=source%2F.git Old. --- diff --git a/biblio/15084599.mdwn b/biblio/15084599.mdwn new file mode 100644 index 00000000..c5394068 --- /dev/null +++ b/biblio/15084599.mdwn @@ -0,0 +1,10 @@ +[[!meta title="RNA substrate specificity and structure-guided mutational analysis of bacteriophage T4 RNA ligase 2."]] +[[!tag enzyme ligase]] + +J Biol Chem. 2004 Jul 23;279(30):31337-47. doi:10.1074/jbc.M402394200 + +Nandakumar J, Ho CK, Lima CD, Shuman S. + +RNA substrate specificity and structure-guided mutational analysis of bacteriophage T4 RNA ligase 2. + +[[!pmid 15084599 desc="Can ligate RNA at a 3'-OH/5'-P nick in a RNA/RNA or RNA/DNA duplex."]] diff --git a/biblio/15851476.mdwn b/biblio/15851476.mdwn new file mode 100644 index 00000000..882afcf3 --- /dev/null +++ b/biblio/15851476.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Dual mechanisms whereby a broken RNA end assists the catalysis of its repair by T4 RNA ligase 2."]] +[[!tag enzyme ligase]] + +Nandakumar J, Shuman S. + +J Biol Chem. 2005 Jun 24;280(25):23484-9. doi:10.1074/jbc.M500831200 + +Dual mechanisms whereby a broken RNA end assists the catalysis of its repair by T4 RNA ligase 2. + +[[!pmid 15851476 desc="Complete Appylation by RNL2 is possible in absence of Mg2+, but is slower."]] diff --git a/biblio/To_Do b/biblio/To_Do index bf358a97..d678dcf1 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -332,9 +332,6 @@ Alternative promoters which transcribe variants with different translational eff 15342556 [genome] These blocks contain most validated and predicted transcription factor binding sites. -15084599 [enzymes] -Further characterisation of the enzyme. - 15509747 [visual cortex] Another slightly different critical period, and another PV:GFP transgene. @@ -977,9 +974,6 @@ Breadth-first search: organises a network in a layered hierarchy. Highest nodes 16893951 [amplification] Combined use of T7 polymerase and helicase, strong strand displacement, and branched rolling circle isothermal amplification. -15084599 [enzymes] -Complete Appylation by RNL2 is possible in absence of Mg2+, but is slower. - 16964210 [amplification] Apparently, DNA - RNA hybridisation is very tolerant to mismatches.