From: Charles Plessy Date: Thu, 12 Oct 2017 04:04:20 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=92a783c7389d38474c0d7b7bb57b4fb2003c5b51;p=source%2F.git Old --- diff --git a/biblio/16791213.mdwn b/biblio/16791213.mdwn index 1fe192e1..25fa5219 100644 --- a/biblio/16791213.mdwn +++ b/biblio/16791213.mdwn @@ -1,3 +1,9 @@ [[!meta title="Amplification of complex gene libraries by emulsion PCR."]] -[[!tag emulsion]] +[[!tag emulsion PCR]] + +Nat Methods. 2006 Jul;3(7):545-50 doi:10.1038/nmeth896 + +Williams R, Peisajovich SG, Miller OJ, Magdassi S, Tawfik DS, Griffiths AD. + +Amplification of complex gene libraries by emulsion PCR. [[!pmid 16791213 desc="Protects the templates frome size bias by isolating them from each other."]] diff --git a/biblio/16791214.mdwn b/biblio/16791214.mdwn index 437bfcfb..80996275 100644 --- a/biblio/16791214.mdwn +++ b/biblio/16791214.mdwn @@ -1,3 +1,10 @@ [[!meta title="BEAMing: single-molecule PCR on microparticles in water-in-oil emulsion."]] [[!tag emulsion biotin]] + +Nat Methods. 2006 Jul;3(7):551-9 doi:10.1038/nmeth898 + +Diehl F, Li M, He Y, Kinzler KW, Vogelstein B, Dressman D + +BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions. + [[!pmid 16791214 desc="Recommends (in Box 1) a double-biotin to avoid breaking the complexes at high temperatures."]] diff --git a/biblio/16791215.mdwn b/biblio/16791215.mdwn index 791dd70d..7dfc2747 100644 --- a/biblio/16791215.mdwn +++ b/biblio/16791215.mdwn @@ -1,3 +1,10 @@ [[!meta title="Directed evolution by in vitro compartmentalization."]] [[!tag emulsion]] + +Nat Methods. 2006 Jul;3(7):561-70 doi:10.1038/nmeth897 + +Miller OJ, Bernath K, Agresti JJ, Amitai G, Kelly BT, Mastrobattista E, Taly V, Magdassi S, Tawfik DS, Griffiths AD. + +Directed evolution by in vitro compartmentalization. + [[!pmid 16791215 desc="Method for water in oil in water emulsion (w/o/w)."]] diff --git a/tags/emulsion.mdwn b/tags/emulsion.mdwn index 5e2895f5..4771a2ca 100644 --- a/tags/emulsion.mdwn +++ b/tags/emulsion.mdwn @@ -8,5 +8,6 @@ How to break the droplets ? - In [[Nakano _et al._, 2005|biblio/16233792]], by centrifugation (oil: silicone; superfactant: TritonX-100 0.1%). - In [[Agresti et al., 2005|biblio/16260754]], by centrifugation in presence of diethyl ether (oil: mineral; superfactant: TritonX-100 0.1% and Span 80 4.5%). + - In [[Dielh _et al._, 2006|biblio/16791214]], by pipetting up and down in presence of high salt (100 mM NaCl), SDS (1%), TritonX-100 (1%) (oil: mineral, superfactant: ABIL WE09). [[!inline pages="tagged(emulsion)" actions="no" feedshow=0]]