From: admin Date: Mon, 15 Jun 2020 07:41:09 +0000 (+0000) Subject: updated PO files X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=88a5c0eadbfd9308a28a81798e37a82b4e584809;p=source.git updated PO files --- diff --git a/open-source-biologist.en.po b/open-source-biologist.en.po new file mode 100644 index 00000000..9a9a3dce --- /dev/null +++ b/open-source-biologist.en.po @@ -0,0 +1,104 @@ +# SOME DESCRIPTIVE TITLE +# Copyright (C) YEAR Free Software Foundation, Inc. +# This file is distributed under the same license as the PACKAGE package. +# FIRST AUTHOR , YEAR. +# +#, fuzzy +msgid "" +msgstr "" +"Project-Id-Version: PACKAGE VERSION\n" +"POT-Creation-Date: 2020-06-15 07:41+0000\n" +"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n" +"Last-Translator: FULL NAME \n" +"Language-Team: LANGUAGE \n" +"Language: \n" +"MIME-Version: 1.0\n" +"Content-Type: text/plain; charset=UTF-8\n" +"Content-Transfer-Encoding: 8bit\n" + +#. type: Plain text +msgid "" +"My training as a researcher started with developmental genetics in " +"drosophila and zebrafish, where I studied the activity of transcription " +"enhancers (Blader et al., 2003) and their evolutionary conservation (Plessy " +"et al., 2005). This gave me a strong interest for whole-transcriptome " +"analysis and technology. For that purpose, I have joined RIKEN in 2004, " +"where have worked on high-throughput methods for profiling promoters and " +"inferring gene networks, and in particular on CAGE (Cap Analysis Gene " +"Expression)." +msgstr "" + +#. type: Plain text +msgid "" +"I have developed a miniaturized version of CAGE, termed nanoCAGE, to analyse " +"small samples yielding only nanograms of RNA (Plessy et al., 2010). In the " +"same manuscript, we also introduced its paired-end variant, CAGEscan, which " +"we use to associate novel promoters with annotations. Since then, we have " +"kept improving or expanding these techniques, by updating the protocol " +"(Salimullah et al., 2011), reducing the sequence bias introduced by the " +"molecular barcodes (Tang et al., 2013), combining multiple cap-enrichment " +"steps (Batut et al., 2013), benchmarking the use of locked nucleic acids for " +"template switching (Harbers et al., 2013), and reducing the number of primer " +"artefacts and unwanted sequences generated by ribosomal RNAs using " +"low-complexity “pseudo-random” reverse-transcription primers (Arnaud et al., " +"2016)." +msgstr "" + +#. type: Plain text +msgid "" +"On April 2013, I started a new development cycle as the leader of the " +"Genomics Miniaturization Technology Unit at RIKEN Center for Life Sciences, " +"Division of Genomics Technology, to expand this work on single cells " +"following a population transcriptomics approach (Plessy et al., 2013) " +"focused on sampling the largest possible number of cells. In our ongoing " +"developments, we have reached single-cell and single molecule resolution " +"through the introduction of transposase fragmentation and unique molecular " +"identifiers (Poulain et al., 2017). The protocol exists in two versions, one " +"for FACS-isolated cells, and one for the Fluidigm C1 platform (Kouno et al., " +"2019)." +msgstr "" + +#. type: Plain text +msgid "" +"I have complemented my work on CAGE with the development of a gene-centred " +"technique for detecting promoters, termed Deep-RACE (Olivarius et al., 2009, " +"Plessy et al., 2012), which we used to validate our discovery of the " +"pervasive expression of retrotransposons detected by CAGE (Faulkner et al., " +"2009). To study transcription start activity at nucleotide resolution in " +"zebrafish transfected with chimeric transgenes containing a copy of an " +"endogenous promoter, I combined Deep-RACE, CAGE and paired-end sequencing in " +"a technology that we called “Single-Locus CAGE” (Haberle et al., 2014). With " +"my contributions related to CAGE development and analysis, I have been a " +"member of the FANTOM consortium since FANTOM3." +msgstr "" + +#. type: Plain text +msgid "" +"Together with my colleagues at RIKEN and collaborators in the field of " +"neuroscience, I have applied nanoCAGE to the study of single neuron cell " +"types, for instance the olfactory neurons (Plessy et al., 2012), or in " +"dopaminergic cells, where we could demonstrate the expression of haemoglobin " +"in the midbrain (Biagioli et al., 2009). We are also exploring the " +"sub-cellular localisation of RNA in Purkinje neurons (Kratz et al., 2014), " +"and neurogenesis in the mouse olfactory epithelium using single-cell CAGE " +"and ATAC-seq techniques. In parallel with this promoter-centric work, I have " +"also explored the huge repertoire of the T cell antigen receptors." +msgstr "" + +#. type: Plain text +msgid "" +"I joined OIST in 2018, to study the genetic structure and population " +"variations of an animal plankton, Oikopleura dioica, that has a genome 50 " +"time more compact than the human one, which empowers us to sequence at " +"chromosomal resolution many individual sampled from all over the World." +msgstr "" + +#. type: Plain text +msgid "" +"I am also a Free Software enthusiast, and contribute to the Debian Med " +"project, by packaging bioinformatics tools, which are redistributed in " +"Debian (Möller et al., 2010) and its derivatives such as Ubuntu and " +"(cloud)Bio-Linux. For digital signature of my contributions and other " +"activities as a RIKEN researcher, I use the GPG key number B3443334. My " +"ORCID ID is 0000-0001-7410-6295." +msgstr ""