From: Charles Plessy <plessy@riken.jp>
Date: Wed, 14 Feb 2018 07:25:13 +0000 (+0900)
Subject: Old
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=86f6414cb638602f0275533ebfb453b516b096d8;p=source%2F.git

Old
---

diff --git a/biblio/12448877.mdwn b/biblio/12448877.mdwn
index d18baa86..868eb9ba 100644
--- a/biblio/12448877.mdwn
+++ b/biblio/12448877.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Global amplification of cDNA from limiting amounts of tissue. An improved method for gene cloning and analysis"]]
-[[!tag RACE ligase amplification protocol]]
+[[!tag RACE ligase amplification library]]
 
 Mol Biotechnol. 2002 Nov;22(3):223-30 doi:10.1385/MB:22:3:223
 
diff --git a/biblio/16708763.mdwn b/biblio/16708763.mdwn
index ab4171f1..4206c787 100644
--- a/biblio/16708763.mdwn
+++ b/biblio/16708763.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA."]]
-[[!tag protocols cDNA oligonucleotides hybridisation random_priming reverse_transcription]]
+[[!tag library cDNA oligonucleotides hybridisation random_priming reverse_transcription]]
 
 Biotechniques. 2006 May;40(5):649-57.
 
diff --git a/biblio/17124291.mdwn b/biblio/17124291.mdwn
new file mode 100644
index 00000000..d51c322a
--- /dev/null
+++ b/biblio/17124291.mdwn
@@ -0,0 +1,7 @@
+[[!meta title="Distinct populations of primary and secondary effectors during RNAi in C. elegans."]]
+[[!tag sRNA library method]]
+
+
+Distinct populations of primary and secondary effectors during RNAi in C. elegans.
+
+[[!pmid 17124291 desc="5′ppp RNAs cloned by first ligating an adenylylated linker to the 3′ of the RNAs, and then after reverse-transcription ligating another adenylylated linker to the 3′ end of the first-strand cDNA."]]
diff --git a/biblio/To_Do b/biblio/To_Do
index 03a946d9..dba387ff 100644
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -623,9 +623,6 @@ Function of an enhancer lost in teleosts transferred to another one.
 15020752 [tags]
 Rescures circularised concatemers by partial digestion.
 
-17124291 [miRNA]
-5'ppp RNAs cloned using a "ligation independant" protocol.
-
 17158288 [miRNA]
 Small RNA cloned by 3' polyadenylation and 5' RACE.
 
diff --git a/tags/protocol.mdwn b/tags/protocol.mdwn
deleted file mode 100644
index e1f09292..00000000
--- a/tags/protocol.mdwn
+++ /dev/null
@@ -1,4 +0,0 @@
-[[!meta title="pages tagged protocol"]]
-
-[[!inline pages="tagged(protocol)" actions="no" archive="yes"
-feedshow=10]]
diff --git a/tags/protocols.mdwn b/tags/protocols.mdwn
deleted file mode 100644
index e3020ac3..00000000
--- a/tags/protocols.mdwn
+++ /dev/null
@@ -1,4 +0,0 @@
-[[!meta title="pages tagged protocols"]]
-
-[[!inline pages="tagged(protocols)" actions="no" archive="yes"
-feedshow=10]]
diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 8f0d40b9..9d9ac9d8 100644
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -8,10 +8,16 @@ Additives that increase reaction performance:
  - T4 bacteriophage gene 32 protein (T4gp32, [[Kenzelmann _et al._, 2004|biblio/15028277]], [[Piché _et al._, 2005|biblio/16461948]]).
 
 Reverse-transcriptases have a DNA-dependent DNA polymerase activity.
+
  - It is utilised in [[template_switching]] methods to add linkers to first-strand cDNAs.
  - It is also a source of antisense artefacts when the RT makes a second-strand cDNA
    that is mistaken for a first-strand cDNA.  ActinomycinD inhibits DNA-dependent,
    but not RNA-dependent polymerase activity and is used to suppress these
    artefacts ([[Perocchi et al., 2007|biblio/17897965]], [[Kanamori-Katayama et al., 2011|biblio/21596820]]).
 
+Reverse-transcription primers:
+
+ - "N15" random pentadecamers: [[Stangegaard et al., 2006|biblio/16708763]].
+ - ... and many more (not-so-random; pseudo-random, ...)
+
 [[!inline pages="tagged(reverse_transcription)" actions="no" limit=0]]