From: Charles Date: Wed, 13 Nov 2019 07:00:12 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=86dfbaacc99cfcd26d745cc92ca8f514fbb61842;p=source.git Café --- diff --git a/biblio/31543449.mdwn b/biblio/31543449.mdwn new file mode 100644 index 00000000..396eb71a --- /dev/null +++ b/biblio/31543449.mdwn @@ -0,0 +1,17 @@ +[[!meta title="Evolution of the U2 Spliceosome for Processing Numerous and Highly Diverse Non-canonical Introns in the Chordate Fritillaria borealis."]] +[[!tag Oikopleura intron]] + +Curr Biol. 2019 Oct 7;29(19):3193-3199.e4. doi:10.1016/j.cub.2019.07.092 + +Henriet S, Colom Sanmartí B, Sumic S, Chourrout D. + +Evolution of the U2 Spliceosome for Processing Numerous and Highly Diverse Non-canonical Introns in the Chordate Fritillaria borealis. + +[[!pmid 31543449 desc="In Fritillaria borealis, most ancestral canonical +introns were lost. New introns with non-canonical splice sites were created by +the insertion of MITEs (miniature inverted-repeat transposable elements) after +TA sites. Splicing of non-canonical introns can be inhibited with Pladienolide +B. Non-canonical introns of F. bor and O. dioica genes can not be spliced in +HEK293T cells, but canonical introns can. In ~90% of lariats from F. bor or O. +dio, the branchpoint is an A. Distance to 3′ splice site is similar in both +species. A single U2 spliceosome was found in F. bor."]] diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn index 752a59d6..1259ee44 100644 --- a/tags/Oikopleura.mdwn +++ b/tags/Oikopleura.mdwn @@ -255,12 +255,17 @@ Transcriptome - “Maternal promoters in O. dioica were located on the X-chromosome more frequently than expected” ([[Danks et al, 2018|biblio/29482522]]). - Introns are very small (peak at 47 base pairs, 2.4% > 1 kb, [[Denoeud et - al., 2010|biblio/21097902]]) and subjected to a large turnover: many ancestral - introns are lost, and many new species-specific introns found ([[Edvarsen et - al., 2004|biblio/15638456]]). Introns are gained by insertion of transposon-like elements and - by reverse splicing, ([[Denoeud et al., 2010|biblio/21097902]]). Larger introns tend - to be older, and among the large introns, the older contain repeat elements less frequently - than the newer ([[Denoeud et al., 2010|biblio/21097902]]). + al., 2010|biblio/21097902]]) and subjected to a large turnover: many + ancestral introns are lost, and many new species-specific introns found + ([[Edvarsen et al., 2004|biblio/15638456]]). Introns are gained by insertion + of transposon-like elements and by reverse splicing, ([[Denoeud et al., + 2010|biblio/21097902]]). Larger introns tend to be older, and among the large + introns, the older contain repeat elements less frequently than the newer + ([[Denoeud et al., 2010|biblio/21097902]]). In _Fritilaria borealis_, most + introns are derived from the insertion of MITE elements and are non-canonical + ([[Henriet and coll., 2019|biblio/31543449]]). + - HEK293T cells can only splice canonical _O. dioica_ (and _F. borealis_) introns, + but not the non-canonical ones ([[Henriet and coll., 2019|biblio/31543449]]). - 12 developmental stages were studied with tiling arrays by [[Danks et al., 2013|biblio/23185044]]. - On the histone mRNAs, no purine-rich histone downstream element (HDE) was found by [[Chioda, Eskeland and Thompson in 2002|biblio/12446815]].